The Effect of Sodium Valproate and Low Frequency Electrical Stimulation on Motor Activity During Rapid Kindling of Hippocampal CA1 Region in Adult Male Rat: An Experimental Study

Background and Objectives: Epilepsy is a chronic neurological disorder that requires long treatment with less side effects. Therefore, this study aimed to determine the effect of sodium valproate and electrical stimulation of the brain with low frequency (LFS) on the motor activity of male rat during hippocampus rapid kindling. Material and Methods: In this experimental study, 56 male adult rats were randomly divided to 8 groups: control, sham operation, saline-kindled and drug-kindled groups which received saline or drug 15 minutes before kindling stimulations and saline-kindled-LFS or drug-kindled-LFS group which received saline or drug 15 minutes before kindling stimulations and LFS after termination of kindling stimulations. Then animals motor activity was assessed on the 6th day by open field test and data were analyzed by one-way ANOVA and Tukey's post-test. Results: The open field results showed a significant increase in the number of motions (p =0.007) in the kindled group compared to the sham group. But these parameters showed no difference in saline-kindled-LFS group, drug-kindled and drug-kindled-LFS groups compared with the sham group (p˃0.05). Conclusion: It seems administration of sodium valproate and application of low-frequency electrical to hippocampus region can attenuate the kindling-induced hyperactivity in adult male rat. Key­ words: Sodium valproate, Low frequency electrical stimulation, Open field, Kindling, Rat   Funding: This research was funded by Shahid Chamran University of Ahvaz. Conflict of interest: None declared. Ethical approval: The Ethical Committee of Shahid Chamran University of Ahvaz approved the study (EE/97.3.24.49755/scu.ac.ir).   How to cite this article: Zalkhani R, Moazedi AA, Ghotbeddin Z, Pourmahdi Borujeni M. The Effect of Sodium Valproate and Low Frequency Electrical Stimulation on Motor Activity During Rapid Kindling of Hippocampal CA1 Region in Adult Male Rat: An Experimental Study. J Rafsanjan Univ Med Sci 2019; 18 (1): 43-54. [Farsi]